Determination of long-chain base in glycosphingolipids with fluorescamine

Determination of long-chain base in glycosphingolipids with fluorescamine

A method is described for determining the long-chain base content of glycosphingolipids after acid hydrolysis, using the new reagent fluorescamine. The reaction is sensitive and can be used to characterize or measure glycosphingolipids in quantities routinely separated by thin-layer chromatography.

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Bibliographische Detailangaben
Veröffentlicht in:Journal of lipid research 1974-03, Vol.15 (2), p.179-180
Hauptverfasser: Kisic, A, Rapport, M M
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Benzofurans
Brain
Cattle
Ceramides - analysis
Chromatography, Thin Layer
cytolipin H
cytolipin K
cytolipin R
Evaluation Studies as Topic
fluorescence
Galactose - analysis
galactosyl ceramide
Gangliosides - analysis
Humans
Indicators and Reagents
Kidney
Methods
Microchemistry
Molecular Weight
Neuraminidase
Spectrometry, Fluorescence
Sphingolipids - analysis
sphingosine
Spiro Compounds
Tritium
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Beschreibung
Zusammenfassung:A method is described for determining the long-chain base content of glycosphingolipids after acid hydrolysis, using the new reagent fluorescamine. The reaction is sensitive and can be used to characterize or measure glycosphingolipids in quantities routinely separated by thin-layer chromatography.
ISSN:0022-2275
DOI:10.1016/S0022-2275(20)36822-X