Development of a CRISPR-Cas12a system for efficient genome engineering in clostridia
Continued efforts in developing the CRISPR-Cas systems will further enhance our understanding and utilization of species. This study demonstrates the development and application of a genome-engineering tool in two strains, and , which have promising potential as probiotics and oncolytic agents. Part...
Gespeichert in:
Veröffentlicht in: | Microbiology spectrum 2023-12, Vol.11 (6), p.e0245923-e0245923 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Continued efforts in developing the CRISPR-Cas systems will further enhance our understanding and utilization of
species. This study demonstrates the development and application of a genome-engineering tool in two
strains,
and
, which have promising potential as probiotics and oncolytic agents. Particular attention was given to the folding of precursor crRNA and the role of this process in off-target DNA cleavage by Cas12a. The results provide the guidelines necessary for efficient genome engineering using this system in clostridia. Our findings not only expand our fundamental understanding of genome-engineering tools in clostridia but also improve this technology to allow use of its full potential in a plethora of biotechnological applications. |
---|---|
ISSN: | 2165-0497 2165-0497 |
DOI: | 10.1128/spectrum.02459-23 |