RNA G-quadruplex structures exist and function in vivo in plants

Guanine-rich sequences are able to form complex RNA structures termed RNA G-quadruplexes in vitro. Because of their high stability, RNA G-quadruplexes are proposed to exist in vivo and are suggested to be associated with important biological relevance. However, there is a lack of direct evidence for...

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Veröffentlicht in:Genome Biology 2020-09, Vol.21 (1), p.226-226, Article 226
Hauptverfasser: Yang, Xiaofei, Cheema, Jitender, Zhang, Yueying, Deng, Hongjing, Duncan, Susan, Umar, Mubarak Ishaq, Zhao, Jieyu, Liu, Qi, Cao, Xiaofeng, Kwok, Chun Kit, Ding, Yiliang
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Sprache:eng
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Zusammenfassung:Guanine-rich sequences are able to form complex RNA structures termed RNA G-quadruplexes in vitro. Because of their high stability, RNA G-quadruplexes are proposed to exist in vivo and are suggested to be associated with important biological relevance. However, there is a lack of direct evidence for RNA G-quadruplex formation in living eukaryotic cells. Therefore, it is unclear whether any purported functions are associated with the specific sequence content or the formation of an RNA G-quadruplex structure. Using rG4-seq, we profile the landscape of those guanine-rich regions with the in vitro folding potential in the Arabidopsis transcriptome. We find a global enrichment of RNA G-quadruplexes with two G-quartets whereby the folding potential is strongly influenced by RNA secondary structures. Using in vitro and in vivo RNA chemical structure profiling, we determine that hundreds of RNA G-quadruplex structures are strongly folded in both Arabidopsis and rice, providing direct evidence of RNA G-quadruplex formation in living eukaryotic cells. Subsequent genetic and biochemical analyses show that RNA G-quadruplex folding is able to regulate translation and modulate plant growth. Our study reveals the existence of RNA G-quadruplex in vivo and indicates that RNA G-quadruplex structures act as important regulators of plant development and growth.
ISSN:1474-760X
1474-7596
1474-760X
DOI:10.1186/s13059-020-02142-9