Genome-Wide Analysis and Expression of Cyclic Nucleotide-Gated Ion Channel ( CNGC ) Family Genes under Cold Stress in Mango ( Mangifera indica )

The 'king of fruits' mango ( ) is widely cultivated in tropical areas and has been threatened by frequent extreme cold weather. ( ) genes have an important function in the calcium-mediated development and cold response of plants. However, few -related studies are reported in mango, regardless of the mango cold stress response. In this study, we identified 43 genes in mango showing tissue-specific expression patterns. Five display more than 3-fold gene expression induction in the fruit peel and leaf under cold stress. Among these, and are significantly upregulated below 6 °C, suggesting their candidate functions under cold stress. Furthermore, cell membrane integrity was damaged at 2 °C in the mango leaf, as shown by the content of malondialdehyde (MDA), and eight are positively correlated with MDA contents. The high correlation between and MDA implies might regulate cell membrane integrity by regulating MDA content. Together, these findings provide a valuable guideline for the functional characterization of genes and will benefit future studies related to cold stress and calcium transport in mango.