R(h)oad to antitumour therapy

SEE PDF] Yang et al. showed that in U2OS cells (human bone osteosarcoma epithelial cells) G-Rh2 is responsible for upregulating LC3-II levels, which is further enhanced by the addition of CQ lysosomal inhibitor, proving that G-Rh2 promotes autophagy in osteosarcoma epithelial cells. [...]once these genes are knockdown Rh2-mediated effect is blocked. The unfolded protein response (UPR) main purpose is to restore the ER's homeostasis, however, continual UPR activation can trigger cell death pathways. Additionally, they suggested that the combination of G-Rh2 and MTX also induces apoptosis and it is increased by the lysosomal inhibitor CQ, indicating that the apoptosis pathway is also implicated in antitumour activities. [...]Z-VAD-FMK, an apoptosis inhibitor, constraints apoptosis by G-Rh2 together with MTX, but neither influenced intracellular ATP levels, nor cell surface CALR exposure. [...]the identification of immune cells along with H&E staining of infiltrating immune cells at different time points could further expand on this important observation.