A single-cell landscape of pre- and post-menopausal high-grade serous ovarian cancer ascites

High-grade serous ovarian cancer (HGSOC) is a hormone-related cancer with high mortality and poor prognosis. Based on the transcriptome of 57,444 cells in ascites from 10 patients with HGSOC (including 5 pre-menopausal and 5 post-menopausal patients), we identified 14 cell clusters which were further classified into 6 cell types, including T cells, B cells, NK cells, myeloid cells, epithelial cells, and stromal cells. We discovered an increased proportion of epithelial cells and a decreased proportion of T cells in pre-menopausal ascites compared with post-menopausal ascites. GO analysis revealed the pre-menopausal tumor microenvironments (TME) are closely associated with viral infection, while the post-menopausal TME are mostly related to the IL-17 immune pathway. SPP1/CD44-mediated crosstalk between myeloid cells and B cells, NK cells, and stromal cells mainly present in the pre-menopausal group, while SPP1/PTGER4 -mediated crosstalk between myeloid cells and epithelial cells mostly present in the post-menopausal group. [Display omitted] •Single-cell atlas of pre- and post-menopausal HGSOC ascites•SPP1+ macrophages are related to the prognosis of HGSOC•SPP1/CD44 receptor ligand pairs exist in the pre-menopausal HGSOC ascites cells•SPP1/PTGER4 receptor ligand pairs exist in the post-menopausal HGSOC ascites cells Microenvironment; Cancer