Expression and clinical significance of PD-1, PD-L1 and tumor infiltrating lymphocytes in neuroblastoma

Objective To investigate the expression of programmed cell death protein 1(PD-1), programmed cell death 1 ligand 1 (PD-L1) and tumor infiltrates lymphocytes (TIL) density in neuroblastoma and the relationship between them and the clinicopathological features of patients. Methods The postoperative tumor tissues of 58 patients with neuroblastoma who underwent surgical resection in Shanxi Children ‘s Hospital from January 2000 to June 2022 were selected, and the expression of PD-1 in tumor lymphocytes and PD-L1 in tumor cells were detected by immunohistochemical method. T lymphocyte density of CD3+, CD4+ and CD8+ was detected. The expression of PD-1 and PD-L1 and the relationship between T lymphocyte density of different subgroups and clinicopathological characteristics were analyzed. Results The positive expression rates of PD-1 and PD-L1 protein were 44.8% and 43.1%, respectively. There were significant differences in the expression of PD-L1 in INSS stage, INRGSS stage, pathological type and differentiation