Oxyresveratrol-containing Ramulus mori ethanol extract attenuates acute colitis by suppressing inflammation and increasing mucin secretion

•Oxyresveratrol-containing R. mori ethanol extract (ERMO) reduced inflammation.•ERMO increased mucin synthesis through increased expression of MUC2 and TFF3.•ERMO regenerated intestinal mucus layer in DSS-induced mouse colitis model.•ERMO showed disease-alleviating effects in DSS-induced mouse colitis model.•ERMO attenuated acute colitis by suppressing inflammation and stimulating mucin production. Control of inflammation and mucin expression is important in managing inflammatory bowel disease (IBD). This study investigated the effects of an ethanol extract of Ramulus mori containing oxyresveratrol (ERMO) on inflammation and intestinal mucin production. The anti-inflammatory effect of ERMO was measured both in vitro using lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cell line, and in vivo with a dextran sodium sulfate (DSS)-induced mouse colitis model; its mucin-promoting activity was measured in LS 174T goblet cell line. We found that ERMO significantly reduced inflammatory mediators both cells and mice. In the LS 174T goblet cells, ERMO significantly increased MUC2 and TFF3 mRNA expression in a dose-dependent manner compared with negative control cells. ERMO regenerated intestinal mucus layer and showed disease-alleviating effects in DSS-induced mouse colitis model. In conclusion, ERMO significantly attenuates acute colitis by suppressing inflammation and stimulating mucin production.