VprBP directs epigenetic gene silencing through histone H2A phosphorylation in colon cancer

Histone modification is aberrantly regulated in cancer and generates an unbalanced state of gene transcription. VprBP, a recently identified kinase, phosphorylates histone H2A on threonine 120 (T120) and is involved in oncogenic transcriptional dysregulation; however, its specific role in colon cancer is undefined. Here, we show that VprBP is overexpressed in colon cancer and directly contributes to epigenetic gene silencing and cancer pathogenesis. Mechanistically, the observed function of VprBP is mediated through H2AT120 phosphorylation (H2AT120p)‐driven transcriptional repression of growth regulatory genes, resulting in a significantly higher proliferative capacity of colon cancer cells. Our preclinical studies using organoid and xenograft models demonstrate that treatment with the VprBP inhibitor B32B3 impairs colonic tumor growth by blocking H2AT120p and reactivating a transcriptional program resembling that of normal cells. Collectively, our work describes VprBP as a master kinase contributing to the development and progression of colon cancer, making it a new molecular target for novel therapeutic strategies. VprBP is a recently identified kinase that induces transcriptional silencing of growth suppressive genes to cause cancer. In this study, we demonstrate that VprBP is overexpressed in colon cancer and drives tumorigenic transcription program through H2AT120 phosphorylation. VprBP‐mediated H2AT120 phosphorylation is functionally important, because blocking VprBP kinase activity impedes colonic tumor growth.