Involvement of the Na+/Ca2+ Exchanger in the Automaticity of Guinea-Pig Pulmonary Vein Myocardium as Revealed by SEA0400

We examined the involvement of the Na+/Ca2+ exchanger in the automaticity of the pulmonary vein myocardium with a specific inhibitor, SEA0400. Action potentials were recorded from the myocardial layer of isolated guinea-pig pulmonary vein preparations, and Ca2+ transients were recorded from the card...

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Veröffentlicht in:Journal of Pharmacological Sciences 2009, Vol.110(1), pp.111-116
Hauptverfasser: Namekata, Iyuki, Tsuneoka, Yayoi, Takahara, Akira, Shimada, Hideaki, Sugimoto, Takahiko, Takeda, Kiyoshi, Nagaharu, Midori, Shigenobu, Koki, Kawanishi, Toru, Tanaka, Hikaru
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Sprache:eng
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Zusammenfassung:We examined the involvement of the Na+/Ca2+ exchanger in the automaticity of the pulmonary vein myocardium with a specific inhibitor, SEA0400. Action potentials were recorded from the myocardial layer of isolated guinea-pig pulmonary vein preparations, and Ca2+ transients were recorded from the cardiomyocytes. Spontaneous electrical activity was observed in 17.7% of the preparations, which was inhibited by either SEA0400 or ryanodine. In quiescent preparations, ouabain induced electrical activity and spontaneous Ca2+ transients, which were inhibited by SEA0400, as well as ryanodine. These results provide pharmacological evidence that the Na+/Ca2+ exchanger underlies the automaticity of the pulmonary vein myocardium.
ISSN:1347-8613
1347-8648
DOI:10.1254/jphs.08159SC