MDA-MB-231 Breast Cancer Cells and Their CSC Population Migrate Towards Low Oxygen in a Microfluidic Gradient Device

Most cancer deaths are caused by secondary tumors formed through metastasis, yet due to our limited understanding of this process, prevention remains a major challenge. Recently, cancer stem cells (CSCs) have been proposed as the source of metastases, but only little is known about their migratory b...

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Veröffentlicht in:	International journal of molecular sciences 2018-10, Vol.19 (10), p.3047
Hauptverfasser:	Sleeboom, Jelle J F, Toonder, Jaap M J den, Sahlgren, Cecilia M
Format:	Artikel
Sprache:	eng
Schlagworte:	Breast Neoplasms - metabolism Breast Neoplasms - pathology cancer stem cells Cell Line, Tumor Cell Movement Female Humans Lab-On-A-Chip Devices metastasis Microfluidic Analytical Techniques microfluidics migration Neoplastic Stem Cells - metabolism Oxygen - metabolism oxygen gradient tumor microenvironment
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Zusammenfassung:	Most cancer deaths are caused by secondary tumors formed through metastasis, yet due to our limited understanding of this process, prevention remains a major challenge. Recently, cancer stem cells (CSCs) have been proposed as the source of metastases, but only little is known about their migratory behavior. Oxygen gradients in the tumor have been linked to directional migration of breast cancer cells. Here, we present a method to study the effect of oxygen gradients on the migratory behavior of breast CSCs using a microfluidic device. Our chip contains a chamber in which an oxygen gradient can be generated between hypoxic (
ISSN:	1422-0067 1422-0067
DOI:	10.3390/ijms19103047