Green Synthesis and Antibacterial Activity Studies of Silver Nanoparticles from the Aqueous Extracts of Euphorbia hirta

The aqueous extracts of Euphorbia hirta was used to synthesise silver nanoparticles using bioreduction method. The nanoparticles were characterised by UV Vis spectroscopic analysis, SEM, EDX, AFM, XRD analysis. The silver nanoparticles were also tested for antibacterial activity in Pseudomonas aeruginosa and Bacillus subtilis. The minimum inhibitory concentration for the synthesised nano particles were also tested against the two bacterial species for the least concentration of 0.5[micro]g/mL. The swarming motility assay and protein leakage assay was also tested for the nanoparticle. The silver nanoparticles were found to be much effective. Keywords: Silver nanoparticles, Antibacterial activity, MIC, Protein leakage assay, Euphorbia hirta