Duplex Methylation-Specific Semi-Quantitative Real-Time PCR for Cost-Effective & Time-Efficient Diagnostic Screening of Chromosome 15 and 14 Imprinted Regions

: Our goal was to develop two-tier strategy based on -designed methylation specific-duplex polymerase chain reactions (MS-PCRs) that could serve as a relatively simple, cost effective, time efficient approach for molecular screening of imprinted regions on chromosomes 15 and 14. : Patients were referred to examination during infancy due to hypotonia and motor development delay. Duplex MS-PCRs were performed that enabled detection of methylated/unmethylated DNA in and CpG islands via plurality of detection channels on PCR instrument Rotor Gene 6000. : Both, copy number variations as well as methylation changes, were revealed by our designed methodology by focusing gene. No imprinting aberrations were yet discovered in gene. Clinical features of the patients were compared. In agree with literature no typical facial features were observed in PWS patient with imprinting defect and AS UPD patient seems to have a relatively better development and language ability in comparison to deletional form of the disease. : In conclusion we were able to establish new, throughput and robust diagnostic approach to PWS/AS.