Comparative transcriptomics of broad‐spectrum and synthetic cannabidiol treated C2C12 skeletal myotubes

Comparative transcriptomics of broad‐spectrum and synthetic cannabidiol treated C2C12 skeletal myotubes

Cannabidiol (CBD) is widely used in sports for recovery, pain management, and sleep improvement, yet its effects on muscle are not well understood. This study aimed to determine the transcriptional response of murine skeletal muscle myotubes to broad‐spectrum CBD and synthetic CBD (sCBD). Differenti...

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Veröffentlicht in:Physiological Reports 2024-09, Vol.12 (18), p.e70059-n/a
Hauptverfasser: Gillham, Scott H., Cole, Paige L., Viggars, Mark R., Nolan, Andy H., Close, Graeme L., Owens, Daniel J.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cannabidiol
Cannabidiol - pharmacology
Cannabinoids
Care and treatment
Cell culture
Cell Line
Cellular stress response
Cytotoxicity
Down-regulation
Endoplasmic reticulum
Enzymes
Ethylenediaminetetraacetic acid
Experiments
Genes
Hemp
Homeostasis
Hypoxia
Marijuana
Metabolism
Mice
Muscle contraction
Muscle Fibers, Skeletal - drug effects
Muscle Fibers, Skeletal - metabolism
Muscles
Musculoskeletal system
Myotubes
Nonsteroidal anti-inflammatory drugs
Original
Oxidative stress
Pain
Polyphenols
Protein folding
Regular Manuscript
RNA
Skeletal muscle
transcriptome
Transcriptome - drug effects
Transcriptomics
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Zusammenfassung:Cannabidiol (CBD) is widely used in sports for recovery, pain management, and sleep improvement, yet its effects on muscle are not well understood. This study aimed to determine the transcriptional response of murine skeletal muscle myotubes to broad‐spectrum CBD and synthetic CBD (sCBD). Differentiated C2C12 myotubes were treated with 10 μM CBD, sCBD, or vehicle control (DMSO) for 24 h before RNA extraction. Poly‐A tail‐enriched mRNA libraries were constructed and sequenced using 2 × 50 bp paired‐end sequencing. CBD and sCBD treatment induced 4489 and 1979 differentially expressed genes (DEGs; p 
ISSN:2051-817X
2051-817X
DOI:10.14814/phy2.70059