Dysbiosis of gut microbiota after cholecystectomy is associated with non‐alcoholic fatty liver disease in mice

Increasing evidence suggests that cholecystectomy is an independent risk factor for non‐alcoholic fatty liver disease (NAFLD). However, the underlying mechanisms that lead to hepatic lipid deposition after cholecystectomy are unclear. In this study, adult male C57BL/6J mice that underwent a cholecystectomy or sham operation were fed either a high‐fat diet (HFD) or a chow diet for 56 days. Significantly increased steatohepatitis, liver/body weight ratio, hepatic triglycerides, and glucose intolerance were observed in postcholecystectomy mice fed the HFD. Notable alterations in the composition of gut microbiota after cholecystectomy were observed in both HFD‐ and chow‐diet‐fed mice. Our results indicate that cholecystectomy alters the gut microbiota profile, which might contribute to the development of NAFLD in mice. Our study showed that cholecystectomy aggravated metabolic disorder and steatohepatitis induced by HFD, which were accompanied by a dramatically altered composition of gut microbiota. These results suggest that gut microbiota disorder caused by cholecystectomy might contribute to the development of NAFLD.