Visualizing Intramolecular Dynamics of Membrane Proteins

Membrane proteins play important roles in biological functions, with accompanying allosteric structure changes. Understanding intramolecular dynamics helps elucidate catalytic mechanisms and develop new drugs. In contrast to the various technologies for structural analysis, methods for analyzing int...

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Veröffentlicht in:International journal of molecular sciences 2022-11, Vol.23 (23), p.14539
Hauptverfasser: Ohkubo, Tatsunari, Shiina, Takaaki, Kawaguchi, Kayoko, Sasaki, Daisuke, Inamasu, Rena, Yang, Yue, Li, Zhuoqi, Taninaka, Keizaburo, Sakaguchi, Masaki, Fujimura, Shoko, Sekiguchi, Hiroshi, Kuramochi, Masahiro, Arai, Tatsuya, Tsuda, Sakae, Sasaki, Yuji C, Mio, Kazuhiro
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Sprache:eng
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Zusammenfassung:Membrane proteins play important roles in biological functions, with accompanying allosteric structure changes. Understanding intramolecular dynamics helps elucidate catalytic mechanisms and develop new drugs. In contrast to the various technologies for structural analysis, methods for analyzing intramolecular dynamics are limited. Single-molecule measurements using optical microscopy have been widely used for kinetic analysis. Recently, improvements in detectors and image analysis technology have made it possible to use single-molecule determination methods using X-rays and electron beams, such as diffracted X-ray tracking (DXT), X-ray free electron laser (XFEL) imaging, and cryo-electron microscopy (cryo-EM). High-speed atomic force microscopy (HS-AFM) is a scanning probe microscope that can capture the structural dynamics of biomolecules in real time at the single-molecule level. Time-resolved techniques also facilitate an understanding of real-time intramolecular processes during chemical reactions. In this review, recent advances in membrane protein dynamics visualization techniques were presented.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms232314539