Detection of titanium particles in human liver and spleen and possible health implications

Titanium dioxide (TiO ) is produced at high volumes and applied in many consumer and food products. Recent toxicokinetic modelling indicated the potential of TiO to accumulate in human liver and spleen upon daily oral exposure, which is not routinely investigated in chronic animal studies. A health risk from nanosized TiO particle consumption could not be excluded then. Here we show the first quantification of both total titanium (Ti) and TiO particles in 15 post-mortem human livers and spleens. These low-level analyses were enabled by the use of fully validated (single particle) inductively coupled plasma high resolution mass spectrometry ((sp)ICP-HRMS) detection methods for total Ti and TiO particles. The presence of TiO in the particles in tissues was confirmed by Scanning Electron Microscopy with energy dispersive X-ray spectrometry. These results prove that TiO particles are present in human liver and spleen, with ≥24% of nanosize (