Preliminary Study on the Mechanism of Chitosan and ε-Polylysine Inhibition against Carbibacterium divergens

To investigate the effects of chitosan and ε-polylysine on the cell structure and cytoprotective enzymes of Carbibacterium divergens to explore their inhibition mechanism against Carbibacterium divergens, the broth dilution method was used to determine the minimum inhibitory concentrations (MICs) of chitosan and ε-polylysine against Carbibacterium divergens. Their effects on the cellular structure of Carbibacterium divergens were then assessed by several indexes, such as growth curves, electronic conductivity, extracellular nucleic acids, extracellular proteins, and alkaline phosphatase (AKP) activity, and the microstructure of bacteria were also observed by scanning electron microscopy (SEM). In addition, their role in oxidative stress was analyzed by detecting changes in catalase (CAT) activity and superoxide dismutase (SOD) activity. The results showed that the MIC of chitosan and ε-polylysine against Carbibacterium divergens was 0.1953 and 0.1563 mg/mL, respectively. The intracellular components (AKP, pro