Clostridioides difficile Infection: Diagnosis and Treatment Challenges

is the most important cause of healthcare-associated diarrhea in the United States. The high incidence and recurrence rates of infection (CDI), associated with high morbidity and mortality, pose a public health challenge. Although antibiotics targeting bacteria are the first treatment choice, antibiotics also disrupt the indigenous gut flora and, therefore, create an environment that is favorable for recurrent CDI. The challenge of treating CDI is further exacerbated by the rise of antibiotic-resistant strains of , placing it among the top five most urgent antibiotic resistance threats in the USA. The evolution of antibiotic resistance in involves the acquisition of new resistance mechanisms, which can be shared among various bacterial species and different strains within clinical and community settings. This review provides a summary of commonly used diagnostic tests and antibiotic treatment strategies for CDI. In addition, it discusses antibiotic treatment and its resistance mechanisms. This review aims to enhance our current understanding and pinpoint knowledge gaps in antimicrobial resistance mechanisms in , with an emphasis on CDI therapies.