PPARγ Regulates Genes Involved in Triacylglycerol Synthesis and Secretion in Mammary Gland Epithelial Cells of Dairy Goats

PPARγ Regulates Genes Involved in Triacylglycerol Synthesis and Secretion in Mammary Gland Epithelial Cells of Dairy Goats

To explore the function of PPARγ in the goat mammary gland, we cloned the whole cDNA of the PPARγ gene. Homology alignments revealed that the goat PPARγ gene is conserved among goat, bovine, mouse, and human. Luciferase assays revealed that rosiglitazone enhanced the activity of the PPARγ response e...

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Veröffentlicht in:PPAR Research 2013-01, Vol.2013 (2013), p.60-69
Hauptverfasser: Shi, Hengbo, Luo, Jun, Zhu, Jiangjiang, Li, Jun, Sun, Yuting, Lin, Xianzi, Zhang, Liping, Yao, Dawei, Shi, Huaiping
Format: Artikel
Sprache:eng
Schlagworte:
Epithelial cells
Gene expression
Genetic aspects
Goats
Health aspects
Physiological aspects
Protein research
Transcription factors
Triglycerides
Zoological research
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Zusammenfassung:To explore the function of PPARγ in the goat mammary gland, we cloned the whole cDNA of the PPARγ gene. Homology alignments revealed that the goat PPARγ gene is conserved among goat, bovine, mouse, and human. Luciferase assays revealed that rosiglitazone enhanced the activity of the PPARγ response element (PPRE) in goat mammary epithelial cells (GMECs). After rosiglitazone (ROSI) treatment of GMECs, there was a significant (P
ISSN:1687-4757
1687-4765
DOI:10.1155/2013/310948