Ammonia oxidizing archaea and bacteria respond to different manure application rates during organic vegetable cultivation in Northwest China

Ammonia oxidization is a critical process in nitrogen cycling that involves ammonia oxidizing archaea (AOA) and bacteria (AOB). However, the effects of different manure amounts on ammonia-oxidizing microorganisms (AOMs) over the course of organic vegetables production remains unclear. We used the amoA gene to evaluated AOMs abundance and community structure in organic vegetable fields. Quantitative PCR revealed that AOB were more abundant than AOA. Among them, the amoA copy number of AOB treated with 900 kgN ha −1 was 21.3 times that of AOA. The potential nitrification rate was significantly correlated with AOB abundance ( P