DNA methylation landscape of 16 canine somatic tissues by methylation-sensitive restriction enzyme-based next generation sequencing

DNA methylation landscape of 16 canine somatic tissues by methylation-sensitive restriction enzyme-based next generation sequencing

DNA methylation plays important functions in gene expression regulation that is involved in individual development and various diseases. DNA methylation has been well studied in human and model organisms, but only limited data exist in companion animals like dog. Using methylation-sensitive restrict...

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Veröffentlicht in:Scientific reports 2021-05, Vol.11 (1), p.10005-10005, Article 10005
Hauptverfasser: Yamazaki, Jumpei, Matsumoto, Yuki, Jelinek, Jaroslav, Ishizaki, Teita, Maeda, Shingo, Watanabe, Kei, Ishihara, Genki, Yamagishi, Junya, Takiguchi, Mitsuyoshi
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Sprache:eng
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631/337/176
631/337/176/1988
631/601
CpG islands
Deoxyribonucleic acid
DNA
DNA methylation
DNA sequencing
Enzymes
Gene expression
Gene mapping
Gene regulation
Humanities and Social Sciences
multidisciplinary
Next-generation sequencing
Principal components analysis
Science
Science (multidisciplinary)
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Zusammenfassung:DNA methylation plays important functions in gene expression regulation that is involved in individual development and various diseases. DNA methylation has been well studied in human and model organisms, but only limited data exist in companion animals like dog. Using methylation-sensitive restriction enzyme-based next generation sequencing (Canine DREAM), we obtained canine DNA methylation maps of 16 somatic tissues from two dogs. In total, we evaluated 130,861 CpG sites. The majority of CpG sites were either highly methylated (> 70%, 52.5–64.6% of all CpG sites analyzed) or unmethylated (
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-89279-0