Anatomical and histochemical aspects of zigotic embryo and leaves in 'Coqueiro Anao'/Aspectos anatomicos e histoquimicos de embriao zigotico e folhas em 'Coqueiro Anao'

The paper pro-vides information about histochemical staining reactions in leaves and embryos of 'Coqueiro Anao' (Cocos nucifera). It was compared in vitro coconut and autotrophic palm leaves. Reactions for insoluble polysaccharides and acidic compounds, protein, extractable lipids, lignin...

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Veröffentlicht in:Ciência rural 2010-04, Vol.40 (4), p.867-872
Hauptverfasser: de Santana, Marlucia Cruz, Souza, Margarete Magalhaes, Pereira, Telma Nair Santana, Teixeira, Silvio Lopes
Format: Artikel
Sprache:eng ; spa
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Zusammenfassung:The paper pro-vides information about histochemical staining reactions in leaves and embryos of 'Coqueiro Anao' (Cocos nucifera). It was compared in vitro coconut and autotrophic palm leaves. Reactions for insoluble polysaccharides and acidic compounds, protein, extractable lipids, lignin and other classes of compounds were tested using histochemical tests. None sample gave positive reaction for lignin and phenolic compounds. All the samples gave positive reaction for protein, starch and insoluble polysaccharides while acidic compounds were positive only in in vitro leaves. Both in vitro and autotrophic leaves gave positive reaction for lipids showing presence of cuticle even in in vitro leaves. Only autotrophic palm leaves showed idioblasts containing calcium oxalate crystals. Key words: Cocos nucifera, in vitro culture, polysaccharides, protein, lipids. Este trabalho fornece informacoes sobre resultados de testes histoquimicos em folhas e embrioes zigoticos de 'coqueiro anao' (Cocos nucifera). Foram comparadas folhas de palmeiras de cultura in vitro e autotroficas. Reacoes para polissacarideos insoluveis e compostos acidicos, proteinas, lipidios, lignina e outras classes de compostos foram testadas por meio de testes histoquimicos. Nenhuma amostra foi positiva para lignina e compostos fenolicos. Todas as amostras foram positivas para proteina, amido e polissacarideos insoluveis, enquanto compostos acidicos foram observados apenas em folhas cultivadas in vitro. Amostras de folhas in vitro e autotroficas apresentaram reacao positiva para lipidios, demonstrando a presenca de cuticula mesmo em plantulas in vitro. Somente folhas de palmeiras autotroficas apresentaram ideoblastos contendo cristais de oxalato de calcio. Palavras-chave: Cocos nucifera, cultura in vitro, polissacarideos, proteinas, lipidios.
ISSN:0103-8478
1678-4596