Next-generation sequencing of the CHO cell transcriptome

From: 22nd European Society for Animal Cell Technology (ESACT) Meeting on Cell Based Technologies Vienna, Austria 15-18 May 2011 Author details1-Department of Cell Culture Technology, Center of Biotechnology (CeBiTec), Bielefeld University, 33615 Bielefeld, GermanyEMPTY2-Bioinformatics Resource Facility, Center of Biotechnology (CeBiTec), Bielefeld University, 33615 Bielefeld, GermanyEMPTY3-Department of Genome Research and Systems Biology, Center of Biotechnology (CeBiTec), Bielefeld University, 33615 Bielefeld, GermanyEMPTY4-Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, 1180 Vienna, AustriaEMPTY Supplemental Information: Different thresholds were used as evidence for quality: p > 0.05, M = - 1 < M < 1 (pink); p [less-than or equal to] 0.05, -1 < M < 1 (dark blue); p [less-than or equal to] 0.05, M [less-than or equal to] -1 || M [greater-than or equal to] 1, A < 6 (red); p [less-than or equal to] 0.05, M [less-than or equal to] -1 || M [greater-than or equal to] 1, A [greater-than or equal to] 6 (green); p > 0.05, M [less-than or equal to] -1 || M [greater-than or equal to] 1 (light blue). [figure omitted; refer to PDF] This CHO microarray is now available for further experiments and will support transcriptional analysis of CHO cells under process conditions for cell line and process optimization.