VITREOUS BODY STRUCTURES INVESTIGATION USING THE «VITREOCONTRAST» SUSPENSION

Purpose. To develop a new method of vitreous body (VB) preparation with the isolate contrasting and separation of the VB structures (canals, cisterns, and their anastomosis).Material and methods. The research was performed in 20 cadaver donor eyes. The preparation of vitreous body was performed as follows. The circle-wise cutting of sclera was carried out in a 4mm distance from the limbus. The anterior segment of the eye was intact. Then we cut sclera between the rectus muscles, forming scleral tabs except the optic nerve disk and macular area (10-11mm in diameter). The tabs were cut off. Then we formed the choroidal and retinal tabs using the blade and anatomical forceps and cut off them too. We used the Vitreocontrast suspension dyeing for a VB structures contrasting. After it we cut the VB cortex, separated the dyed vitreous body structures, and performed the light microscopy examination.Results. We developed a new method of vitreous body preparation with the intact anterior segment and the area in the posterior pole (10-11mm in diameter), equatorially (15-17mm), in the tab cisterns (8-10mm) in the 21.5-23.5mm axial length of the eye. There was not found any integrity or structural disruption, rupture of vitreous during the preparation. The Vitreokontrast suspension has a high adhesion to the structural elements of vitreous body and allows to separate and to isolate the vitreous body structures (canals, cisterns and their anastomosis). The light microscopy examination showed the presence of canals and cisterns walls. This structures are differ from the vitreous matrix.Conclusion. The new method of VB preparation with a maximum maintenance of its structures (intact anterior segment and a site of posterior pole in diameter of 10-11mm) and the new Vitreocontrast dye allow to contrast all vitreous body structures separately. The analysis of this data will allows to detect the pathological changes of the vitreous, to determine its role in developing of vitreoretinal diseases.