Host Factors Genes BcCLC1 and BcCLC2 Confer Turnip Mosaic Virus Resistance in Non-Heading Chinese Cabbage ( Brassica campestris ssp. chinensis )

Clathrin is an evolutionarily highly conserved evolutionary protein consisting of clathrin light chains (CLC) and clathrin heavy chains (CHC), and these form its basic structure. Clathrin is an important host factor in the process of viral infection. In this study, we cloned the gene and the gene from the '49CX' variety of non-heading Chinese cabbage (NHCC, L. ssp. Makino) and verified their functions. The results showed that was mainly localized in the cytomembrane and cytoplasm, and only a small amount entered the nucleus. encoded a protein comprising 265 amino acids that were distributed in the cytomembrane, nucleus, and cytoplasm. A BiFC assay and yeast two-hybrid (Y2H) analysis showed that ( and ) could interact with several TuMV proteins. We further investigated the mechanism of in regulating TuMV virus infections in NHCC, and observed that gene silencing inhibited TuMV infections and overexpression of in promoted TuMV infections in NHCC. Finally, mutants of homologs of were also screened and subjected to TuMV inoculation tests. In conclusion, we speculate that confer (TuMV) resistance in NHCC by interacting with TuMV proteins to promote the intracellular transport of the virus.