Antioxidant processes involving epicatechin decreased symptoms of pine wilt disease

Since the pine wood nematode (PWN, ) invasion of Northeast China, both symptomatic and asymptomatic PWN carriers have been found. Asymptomatic PWN carriers, which are more dangerous than symptomatic carriers, constitute a source of infection in the following spring. The simultaneous presence of symptomatic and asymptomatic PWN carriers indicates that has different tolerance levels to PWN. In this study, validity of susceptibility testing discovered differential types of including Latent Reservoirs, Low Susceptibles, High Susceptibles and Bell Ringers. Among those types, the Low Susceptibles and Latent Reservoirs were asymptomatic PWN carriers, and Latent Reservoirs were the most dangerous. Transcriptome and metabolomic data showed that 5 genes (3 and 2 gene) involved in the epicatechin (EC) synthesis pathway were significantly upregulated, which increased the content of EC antioxidants in Latent Reservoirs. Hydrogen peroxide (H O ) staining and content determination showed that the hypersensitive response (HR) and H O , which functions as a signaling molecule in systemic acquired resistance, decreased in Latent Reservoirs. However, low contents of EC and high contents of H O were found in the High Susceptibles of . RT-PCR results showed that the expression of and was upregulated together only in Latent Reservoirs. These results show that the susceptibility of to PWN differed among different individuals, although no resistant individuals were found. Latent Reservoirs, in which more PWNs resided without visible symptoms prolonged incubation period, inhibited the symptoms caused by H O because of increased contents of the EC antioxidants.