Novel Molecular Techniques for Identifying Agricultural Microorganisms

Agriculture involves activities aimed at improving soil quality for food production. In this environment, microorganisms play a vital role, positively and/or negatively affecting plant growth. Given this impact, knowing the microbiota associated with agricultural systems and phytopathogens is crucial. The microbial culture method has proven ineffective in identifying microorganisms in agricultural systems, and more effective methods with greater scope for their identification currently exist. This review compiles updated information on new methods for studying microorganisms in the agricultural system, such as metagenomics, and new proposals for microorganism identification methods, such as Raman spectrometry, nanotechnology, and phytopathogen biosensors. In addition, it discusses the strengths and limitations of the new methods for microorganism identification.