Molecular Identification and Drug Susceptibility of Candida Species Isolated from the Skin of Patients with Acne Clinical Sings

Introduction: Acne is a pathological disorder and a chronic inflammation in the sebaceous follicles, and one of the most popular dermatology damages that has affected millions of people worldwide. The aim of this study was to identify Candida species in patients with acne as well as determine the drug susceptibility of these species. Materials & Methods: In this cross-sectional study, 70 clinical specimens were taken from the skin of patients suspected of acne by the sterile swab. Subsequently, the specimens were cultured on Sabouraud Dextrose Agar containing chloramphenicol. The plates were incubated for 48 h at 37˚C. The suspected colonies were investigated through microscopic examination and subsequent passages were evaluated according to standard operating procedures and specification of the type of colony color on CHROMagar for the isolation of the yeast. The sequencing method was utilized (ITS2, ITS4) to approve C.species. In addition, the susceptibility testing was performed to assess drug-resistant isolates C. species based on the clinical and laboratory standards institute method. Findings: In total, 11 C. species were identified and isolated that include 8 C.parapsilosis (72.73%), 1 C.krusei (12.5%), 1 C.lusitaniae (12.5%), 1 C.kefir (12.5%), and a Trichosporon asahi. According to the investigation of C. parapsilosis isolates susceptibility to various concentrations of the anti-fungal agents to isolates Cp1and Cp8, the isolated Cp5 with MIC 50 was equal to 32,0.5,0.25 and with MIC 90 of