Simple Spectrophotometric Method for Analysis of Serum Catalase Activity

Introduction: Catalase is a principal constituent of the antioxidant system that attenuates the oxidative stress, which is ubiquitously associated with several types of pathological disorders. Aim: This paper describes a discontinuous assay for the assessment of catalase activity using the titanium tetrachloride/ sulfuric acid reagent. Materials and Methods: Samples containing catalase are incubated with hydrogen peroxide for three minutes prior to fast mixing of aliquots of the incubation mixtures with titanium tetrachloride/sulfuric acid reagent, which measures remaining hydrogen peroxide (H2 O2 ). Absorbance is then read at 405 nm. Dissociation of hydrogen peroxide is proportional to catalase activity in the used sample. One-way analysis of variance (ANOVA) was used to analyse the resulted data. p-value