Determination of bacterial contamination and evaluation of their antibiotic resistance in different parts of the Hazrat Masoumeh hospital in Qom City

Currently, microbial contamination in different parts of hospitals with opportunistic pathogenic bacteria and their antibiotic resistance are the most important factors in the prevalence of nosocomial infections. The purpose of this study was to isolate and identify contaminating bacteria in medical and non-medical equipment in Hazrate Masoumeh hospital Qom city (May-August2017). Methods: Sampling was done from oncology, infectious, ICU and NICU sections using sterile swabs and transfer in BHI broth. Then collected samples were streaked on Nutrient agar and incubated at 37° C for 24 h .The isolates were identified with phenotypic and biochemical standard tests. Antibiotic resistance of isolates was performed using agar disk diffusion method due to CLSI standards. Result: Out of 84 samples, 77 samples had bacterial contamination and 127 strains were isolated. The prevalence of bacterial isolates were Bacillus subtilis (18.59%), Bacillus cereus (13.18%), Pseudomonas (11.63%), and Staphylococcus epidermidis(11.62%) and Corynebacterium sp. (10.55%), respectively. Among of isolated strains, Pseudomonas aeruginosa, Staphylococcus epidermidis and Bacillus subtilis, had more than antibiotic resistance. The most antibiotic resistance was obtained for penicillin. The most isolates were sensitive to Amikacin ، Gentamycin، Ciprofloxacin. Conclusion: Pseudomonas aeruginosa is one of the most important opportunistic pathogens with multi drug resistant characteristic in nosocomial infections and hospital contamination.