Short-interfering RNAs as antivirals against rabies

This study aimed to test in vitro a RNA-interference based antiviral approach for rabies with short-interfering RNAs (siRNAs) against rabies virus nucleoprotein mRNA. BHK-21 cells were infected with serial dilutions of PV rabies virus strain and transfected with a pool of three siRNAs. Direct immuno...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	The Brazilian journal of infectious diseases 2007-04, Vol.11 (2), p.224-225
Hauptverfasser:	Brandão, Paulo Eduardo, Castilho, Juliana Galera, Fahl, Willian, Carnieli, Jr, Pedro, Oliveira, Rafael de Novaes, Macedo, Carla Isabel, Carrieri, Maria Luiza, Kotait, Ivanete
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals antiviral Cell Line Cricetinae Fluorescent Antibody Technique INFECTIOUS DISEASES rabies Rabies virus - genetics Rabies virus - growth & development RNA, Messenger - genetics RNA, Small Interfering - genetics RNA, Small Interfering - metabolism RNA, Small Interfering - therapeutic use RNA, Viral - genetics siRNA Staining and Labeling Virus Replication - genetics
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	This study aimed to test in vitro a RNA-interference based antiviral approach for rabies with short-interfering RNAs (siRNAs) against rabies virus nucleoprotein mRNA. BHK-21 cells were infected with serial dilutions of PV rabies virus strain and transfected with a pool of three siRNAs. Direct immunofluorescence staining showed a 5-time decrease in virus titer when compared to a non-treated plate, showing a promising new approach to the development of antivirals for rabies treatment.
ISSN:	1413-8670 1678-4391
DOI:	10.1590/s1413-86702007000200011