Prevalence of mutations in genes associated with rifampicin and isoniazid resistance in Mycobacterium tuberculosis clinical isolates

Highlights • Total 558 multidrug resistant, 293 RIF mono resistant and 923 INH mono resistant tuberculosis were detected from the 12,786 MDR suspects samples. • Most frequent mutations were found in the region of MUT: S531L as 50.5% and 55.6% specimens out of 558 MDR-TB and 293 rifampicin monoresistant cases. • The rate of occurrences of mutations was found widely in the Rifampicin Resistant Determination Region of rpoB gene, but the hypervariable regions of 530–533 were alarming in the specification. • Six strains had novel MUT3A (T8C) mutation for isoniazid drug.