CRYOPRESERVATION OF STRAWBERRY SHOOT TIPS BY ENCAPSULATION-DEHYDRATION

CRYOPRESERVATION OF STRAWBERRY SHOOT TIPS BY ENCAPSULATION-DEHYDRATION

In vitro grown strawberry (Fragaria x ananassa Duch.) shoot tips were successfully cryopreserved using an encapsulation-dehydration procedure. Encapsulated shoot tips were precultured on MS medium supplemented with different sucrose concentrations (0.25, 0.5, 0.75 and 1.0 M) for 24 h and dehydrated...

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Veröffentlicht in:Notulae botanicae Horti agrobotanici Cluj-Napoca 2006-08, Vol.34 (1), p.28-33
Hauptverfasser: HALMAGYI, Adela, DELIU, C
Format: Artikel
Sprache:eng
Schlagworte:
conservation
dehydration
Fragaria
liquid nitrogen
shoot tip
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Zusammenfassung:In vitro grown strawberry (Fragaria x ananassa Duch.) shoot tips were successfully cryopreserved using an encapsulation-dehydration procedure. Encapsulated shoot tips were precultured on MS medium supplemented with different sucrose concentrations (0.25, 0.5, 0.75 and 1.0 M) for 24 h and dehydrated (1 to 5 hours) in a sterile laminar air flow prior to direct immersion in liquid nitrogen (-196degree). A maximum of 63% regrowth of cryopreserved shoot tips was obtained following preculture in 0.75 M sucrose solution and 4 h dehydration. Plants produced from control and cryopreserved shoot tips were phenotypically similar.
ISSN:0255-965X
1842-4309
DOI:10.15835/nbha341260