Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation

Nanobodies are single-domain antibodies of camelid origin. We generated nanobodies against the vertebrate nuclear pore complex (NPC) and used them in STORM imaging to locate individual NPC proteins with

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Bibliographische Detailangaben
Veröffentlicht in:	eLife 2015-12, Vol.4, p.e11349-e11349
Hauptverfasser:	Pleiner, Tino, Bates, Mark, Trakhanov, Sergei, Lee, Chung-Tien, Schliep, Jan Erik, Chug, Hema, Böhning, Marc, Stark, Holger, Urlaub, Henning, Görlich, Dirk
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies Antigenic determinants Bacteria Biochemistry Cell Biology Comparative analysis Crosslinked polymers Cysteine Cystine epitope mapping Epitope Mapping - methods Fluorescence Fluorescence microscopy Humans label displacement Macromolecular Substances - immunology Macromolecular Substances - isolation & purification Mass spectrometry Nanobodies nanobody native purification nuclear pore complex Nuclear Pore Complex Proteins - immunology Nuclear Pore Complex Proteins - isolation & purification Optical Imaging - methods Protein binding Proteins Sensors Single-Domain Antibodies - metabolism site-specific Staining and Labeling - methods Tools and Resources Viral antibodies Wildlife conservation
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Beschreibung
Zusammenfassung:	Nanobodies are single-domain antibodies of camelid origin. We generated nanobodies against the vertebrate nuclear pore complex (NPC) and used them in STORM imaging to locate individual NPC proteins with
ISSN:	2050-084X 2050-084X
DOI:	10.7554/elife.11349