Generation of induced pluripotent stem cell line (FMCPGHi001-A) from a 25-year-old Chinese Han healthy male donor

Generation of induced pluripotent stem cell line (FMCPGHi001-A) from a 25-year-old Chinese Han healthy male donor

Peripheral blood mononuclear cells (PBMC) were donated by a healthy 25-year-old Han Chinese man and reprogrammed with human transcription factors (Oct4, Sox2, Klf4, and c-Myc) using CytoTune™-iPS 2.0 Sendai Reprogramming Kit. The pluripotency was confirmed by pluripotency markers. The iPSC lines cou...

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Veröffentlicht in:Stem cell research 2022-04, Vol.60, p.102735-102735, Article 102735
Hauptverfasser: Wang, Tao, Tang, Wenjing, Zeng, Quan, Miao, Shuai, Pi, Chenghui, Li, Yingji, Yue, Wen, Yu, Shengyuan
Format: Artikel
Sprache:eng
Schlagworte:
Adult
Cell Differentiation
Cellular Reprogramming
China
Germ Layers
Humans
Induced Pluripotent Stem Cells - metabolism
Leukocytes, Mononuclear
Male
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Zusammenfassung:Peripheral blood mononuclear cells (PBMC) were donated by a healthy 25-year-old Han Chinese man and reprogrammed with human transcription factors (Oct4, Sox2, Klf4, and c-Myc) using CytoTune™-iPS 2.0 Sendai Reprogramming Kit. The pluripotency was confirmed by pluripotency markers. The iPSC lines could be differentiated into three germ layers and be used as a control in drug development and studies on pathological mechanisms.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2022.102735