Active zone compaction correlates with presynaptic homeostatic potentiation

Neurotransmitter release is stabilized by homeostatic plasticity. Presynaptic homeostatic potentiation (PHP) operates on timescales ranging from minute- to life-long adaptations and likely involves reorganization of presynaptic active zones (AZs). At Drosophila melanogaster neuromuscular junctions,...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Cell reports (Cambridge) 2021-10, Vol.37 (1), p.109770-109770, Article 109770
Hauptverfasser: Mrestani, Achmed, Pauli, Martin, Kollmannsberger, Philip, Repp, Felix, Kittel, Robert J., Eilers, Jens, Doose, Sören, Sauer, Markus, Sirén, Anna-Leena, Heckmann, Manfred, Paul, Mila M.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Neurotransmitter release is stabilized by homeostatic plasticity. Presynaptic homeostatic potentiation (PHP) operates on timescales ranging from minute- to life-long adaptations and likely involves reorganization of presynaptic active zones (AZs). At Drosophila melanogaster neuromuscular junctions, earlier work ascribed AZ enlargement by incorporating more Bruchpilot (Brp) scaffold protein a role in PHP. We use localization microscopy (direct stochastic optical reconstruction microscopy [dSTORM]) and hierarchical density-based spatial clustering of applications with noise (HDBSCAN) to study AZ plasticity during PHP at the synaptic mesoscale. We find compaction of individual AZs in acute philanthotoxin-induced and chronic genetically induced PHP but unchanged copy numbers of AZ proteins. Compaction even occurs at the level of Brp subclusters, which move toward AZ centers, and in Rab3 interacting molecule (RIM)-binding protein (RBP) subclusters. Furthermore, correlative confocal and dSTORM imaging reveals how AZ compaction in PHP translates into apparent increases in AZ area and Brp protein content, as implied earlier. [Display omitted] •HDBSCAN analysis of dSTORM data allows precise determination of AZ nanotopology•AZ compaction correlates with acute and chronic PHP•AZ compaction comprises reduced AZ area and enhanced protein density•Higher protein density equals apparent enlargement in confocal microscopy Applying localization microscopy, Mrestani et al. introduce compaction of presynaptic active zone scaffolds and individual Bruchpilot and RIM-binding protein subclusters correlating with presynaptic homeostatic potentiation. Active zone compaction without protein recruitment enhances local molecule concentrations, which may be misjudged as enlargement by intensity-based imaging approaches.
ISSN:2211-1247
2211-1247
DOI:10.1016/j.celrep.2021.109770