MitoTracker: A useful tool in need of better alternatives

The fluorescence viewing of mitochondria is commonly performed by MitoTracker, a lipophilic cationic dye that is taken up by the mitochondria. In this forum, we highlight several issues that may occur with MitoTracker, including staining of other organelles. Our aim is to offer alternative dyes and...

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Veröffentlicht in:	European journal of cell biology 2023-12, Vol.102 (4), p.151371-151371, Article 151371
Hauptverfasser:	Neikirk, Kit, Marshall, Andrea G., Kula, Bartosz, Smith, Nathan, LeBlanc, Sharonda, Hinton, Antentor
Format:	Artikel
Sprache:	eng
Schlagworte:	Fluorescent probes Membrane potential Mitochondria MitoTracker
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	The fluorescence viewing of mitochondria is commonly performed by MitoTracker, a lipophilic cationic dye that is taken up by the mitochondria. In this forum, we highlight several issues that may occur with MitoTracker, including staining of other organelles. Our aim is to offer alternative dyes and discuss their advantages and disadvantages. We also offer options for software with alternatives to MitoTracker to expedite future experimental design.
ISSN:	0171-9335 1618-1298
DOI:	10.1016/j.ejcb.2023.151371