The antibiotic erythromycin did not affect micronucleus frequency in human PHA-stimulated lymphocytes

The aim of the present study was to investigate the ability of the antibiotic erythromycin to induce micro?nucleus (MN) frequency and the cytokinesis block proliferation index (CBPI) in human peripheral blood lymphocytes in vitro using the cytokinesis-block micronucleus test (CBMN). Cell cultures were treated with five different concentrations of erythromycin ranging from 0.68 x 10-4 to 5.45 x 10-4 M. The positive control cells were treated with a known mutagen, mitomycin C (MMC), in a concentration of 1.6 x 10-6 M. None of the tested concentrations of erythromycin significantly changed the average MN frequency or CBPI in PHA-stimulated lymphocytes (p > 0.05). U ovoj studiji je ispitivana sposobnost antibiotika eritromicina da indukuje mikronukleusnu (MN) frekvencu i citokinezis blok proliferacioni indeks (CBPI) u humanim limfocitima periferne krvi in vitro, pomocu citokinezis-blok mikronukleusnog testa (CBMH). Celijske kulture su tretirane sa pet razlicitih koncentracija eritromicina od 0,68 x 10-4 do 5,45 x 10-4 M. Pozitivne kontrolne celije su tretirane poznatim mutagenom, mitomicinom C (MMC) u koncentraciji 1,6 x 10-6 M. Sve testirane koncentracije eritromicina nisu statisticki znacajno menjale prosecnu MN frekvencu i CBPI u PHA stimulisanim limfocitima (p > 0,05).