HPLC methods for the determinatiuon of acetyl- and benzoyl-tiazofurin in rat plasma

Reverse-phase HPLC methods for determination of 5’-O-acetyl-tiazofurin (AT) and 5’-O-benzoyl-tiazofurin (BT) in rat plasma were developed and validated in terms of specificity, linearity, precision, accuracy and sensitivity. Linear calibration curves were constructed in the concentration range of 2.50 – 100.00 μmol/L for both compounds. The separations were achieved on a Supelcosyl LC-18-DB analytical column (250x4.6 mm; 5_μm particle size) by isocratic elution, with a mixture of 0.1Mdisodium hydrogen phosphate – methanol. UV detection was performed at a wavelength of 254 nm. The proposed methods enable the detection and quantification of nanomolar concentrations of AT and BT in rat plasma.