Physico-chemical and functional characteristics of neem seed protein isolate

The demands for plant-based proteins have increased, hence, the search for alternative sources for beneficial purposes. The physico-chemical and functional characteristics of protein isolated from neem seed were ascertained in this work. Neem seed protein isolate (NSPI) contained 86.33% protein rich in charged amino acids. Gel electrophoresis revealed that NSPI consists of polypeptides ranging from 97 to 21 kDa with 28–23 kDa components being prominent. Secondary structure data showed that β–sheets (45.83%) and α–helices (20.42%) were predominant in NSPI. The intrinsic fluorescence emission and surface hydrophobicity data indicated that at pH 3.0, NSPI was highly denatured and has less folded conformation. At pH 3.0 and 9.0, NSPI exhibited the highest protein solubility. NSPI had significantly (p