Pomegranate Seed Oil Shows Inhibitory Effect on Invasion of Human Breast Cancer Cell Lines

Introduction: The pomegranate seeds are a good source of compounds such as conjugated linoleic acid and punicic acid that can have anticancer effects. Aim: To assess the potential inhibitory effects of Pomegranate Seed Oil (PSO) on human breast cancer cell lines metastasis. Materials and Methods: This research was an in vitro study on human breast cancer cell lines MCF-7 and MDA-MB-468. Pomegranate was collected from Fars Province of Iran and the seed oil was extracted. Cells were treated with different concentrations of the oil and their viability was determined using MTT assay. The morphology, colony formation, invasion, migration, wound healing and cell adhesion of cancer cell lines were evaluated. Half maximal inhibitory concentration (IC50) was obtained after 24, 48 and 72hrs. of treatment of cell lines. The statistical analysis of results were done by SPSS 19.0 software using One-way ANOVA followed by Post-hoc Tukey test and the level of significance was set at p