Effect of ascorbic acid, α-tocopherol and its combination on human umbilical vein endothelial cells permeability exposed to high concentration of glucose

Aim — To evaluate the effect of ascorbic acid, α-tocopherol, and its combination on the permeability of human umbilical vein endothelial cells (HUVEC’S) culture after high glucose concentration exposure. Material and Methods — The study was performed using HUVEC’S culture on transwell polyester filt...

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Veröffentlicht in:Russian open medical journal 2020-06, Vol.9 (2), p.e0201
Hauptverfasser: Purnomo, Yudi, Soeatmadji, Djoko W., Widodo, M. Aris
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Sprache:eng
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Zusammenfassung:Aim — To evaluate the effect of ascorbic acid, α-tocopherol, and its combination on the permeability of human umbilical vein endothelial cells (HUVEC’S) culture after high glucose concentration exposure. Material and Methods — The study was performed using HUVEC’S culture on transwell polyester filter. The ascorbic acid, α-tocopherol, and its combination (25-200 µM) were concomitantly given with glucose 22 µM to the culture media. After seven days of treatment, endothelial permeability was observed by measuring the concentration of trypan blue-labeled albumin in a luminal compartment with a spectrophotometer (λ=592 nm). The morphology of the cells was observed under an inverted microscope. The data was analyzed using ANOVA test followed by Dunnet test (p
ISSN:2304-3415
2304-3415
DOI:10.15275/rusomj.2020.0201