Experimental 70% Hepatectomy Model: Apoptotic Index, Proliferative Index and Mitotic Index

Liver regeneration is known to begin after experimentally induced liver resection. At first 24-48 hours, DNA synthesis reaches the maximum level after resection and significantly regeneration known to occur on the first 10 days. In this study, we was examined the regeneration of liver on the 1st and 14th days after the resection. In experiment 21 Wistar albino male rats were used. The rats were randomly divided into three groups. All experimentally groups realized midline incision with laparotomy for resection of liver. Pedicles of the left lateral and median lobes of the liver were applied 70% hepatectomy by 4/0 silk binding. At the end of 1st and 14th days, liver tissue removed for light misroscobic analysis. The tissue of all experimentally groups were showed some histopatological changes such as sinuzoidal dilatation, vacuolization in the hepatocytes. This histopatological findings were seem to increase at II. grup more than experimentally groups. Mitotic index, apoptotic index and proliferation index of values at II. group was reached the maximum level. Other group (III) dramatically reduced the value of these index and were seem to reach near to values of the control group. Relative liver weight that determined each of experimentally groups were statistically significant differences compared to the control group.