Association of Vitamin E with Rapid Thawing on Goat Semen

The aim of this study was to evaluate the effects of vitamin E associated with rapid thawing on cryopreserved goat semen. Two bucks were used and eight ejaculates per animal were collected using artificial vagina. Semen was diluted with the following treatments: BIOXCELL (control), BIOXCELL + Equex...

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Veröffentlicht in:TheScientificWorld 2014-01, Vol.2014 (2014), p.1-5
Hauptverfasser: Torres, Ciro Alexandre Alves, Silveira, Camila Oliveira, Oliveira, Gisele Dias, Dias, Júlio César Oliveira, Carrascal, Erly, Jimenez, Carolina Rodriguez, Oliveira, Fabrício Albani, Penitente-Filho, Jurandy Mauro, Silveira, Renata Gomes
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Sprache:eng
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Zusammenfassung:The aim of this study was to evaluate the effects of vitamin E associated with rapid thawing on cryopreserved goat semen. Two bucks were used and eight ejaculates per animal were collected using artificial vagina. Semen was diluted with the following treatments: BIOXCELL (control), BIOXCELL + Equex (sodium lauryl sulphate) and BIOXCELL + vitamin E 100 μM. Semen was packaged into 0.25 mL straws and cooled at 5°C for 1 hour. Freezing was performed in liquid nitrogen vapor (−155°C) during 15 minutes. Then, the straws were immersed in liquid nitrogen (−196°C). Straws were thawed at 38°C/60 seconds or at 60°C/7 seconds with immediate sperm analysis. Hypoosmotic swelling test was performed adding a 20 μL aliquot of thawed semen to 1 mL of hypoosmotic solution (100 mOsm·Kg−1) followed by incubation during 60 minutes in water bath (38°C). Vitamin E did not affect any studied parameters ( P > 0.05 ). Nevertheless, defrosting rate of 60°C/7 seconds improved sperm membrane functional integrity ( P < 0.05 ). Current knowledge about goat semen cryopreservation is not sufficient to ensure high post-thawing recovery rates; thus, this study brings important data about using antioxidants and different thawing rates on cryopreservation process.
ISSN:2356-6140
1537-744X
1537-744X
DOI:10.1155/2014/964172