Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements

In this study, we show the repetitive detection of toluene on a tapered optical fiber element (OFE) with an attached layer of TVA8 bioluminescent bioreporters. The bioluminescent cell layer was attached on polished quartz modified with (3-aminopropyl)triethoxysilane (APTES). The repeatability of the preparation of the optical probe and its use was demonstrated with five differently shaped OFEs. The intensity of measured bioluminescence was minimally influenced by the OFE shape, possessing transmittances between 1.41% and 5.00%. OFE probes layered with TVA8 were used to monitor liquid toluene over a two-week period. It was demonstrated that OFE probes layered with positively induced TVA8 bioreporters were reliable detectors of toluene. A toluene concentration of 26.5 mg/L was detected after