Quantification of Colibactin-associated Genotoxicity in HeLa Cells by In Cell Western (ICW) Using γ -H2AX as a Marker

Quantification of Colibactin-associated Genotoxicity in HeLa Cells by In Cell Western (ICW) Using γ -H2AX as a Marker

The genotoxin colibactin is produced by several species of . This genotoxin induces DNA damage, cell cycle arrest, senescence and death in eukaryotic cells ( Nougayrède , 2006 ; Taieb , 2016 ). Here we describe a method to quantify the genotoxicity of bacteria producing colibactin following a short...

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Veröffentlicht in:Bio-protocol 2018-03, Vol.8 (6), p.e2771-e2771
Hauptverfasser: Tronnet, Sophie, Oswald, Eric
Format: Artikel
Sprache:eng
Schlagworte:
Biology
Human health and pathology
Life Sciences
Methods
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Zusammenfassung:The genotoxin colibactin is produced by several species of . This genotoxin induces DNA damage, cell cycle arrest, senescence and death in eukaryotic cells ( Nougayrède , 2006 ; Taieb , 2016 ). Here we describe a method to quantify the genotoxicity of bacteria producing colibactin following a short infection of cultured mammalian cells with colibactin producing .
ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.2771