Polyubiquitin Promoter-Based Binary Vectors for Overexpression and Gene Silencing in Lotus japonicus

In this study, we compared the transcriptional activities between Cauliflower mosaic virus (CaMV)35S promoter and polyubiquitin (Ljubq1) promoter from Lotus japonicus using β-glucuronidase (gus) reporter gene in transgenic plants of L. japonicus. The promoter analysis demonstrated that the Ljubq1 pr...

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Veröffentlicht in:	Molecular plant-microbe interactions 2008-04, Vol.21 (4), p.375-382
Hauptverfasser:	Maekawa, T, Kusakabe, M, Shimoda, Y, Sato, S, Tabata, S, Murooka, Y, Hayashi, M
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions beta-glucuronidase Biological and medical sciences Cauliflower mosaic virus Caulimovirus - genetics Economic plant physiology forage crops forage legumes Fundamental and applied biological sciences. Psychology Gene Silencing genetic vectors Genetic Vectors - genetics leaves Loteae - cytology Loteae - genetics Lotus corniculatus var. japonicus Lotus japonicus Models, Genetic molecular sequence data Phytopathology. Animal pests. Plant and forest protection Plant Leaves - genetics Plant Roots - genetics Plant Stems - genetics Plant viruses and viroids Plants, Genetically Modified pollen Pollen - genetics polyubiquitin Polyubiquitin - genetics promoter regions Promoter Regions, Genetic - genetics reporter genes RNA interference root nodules Root Nodules, Plant - genetics roots stems Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) transcription (genetics) transgenic plants
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Zusammenfassung:	In this study, we compared the transcriptional activities between Cauliflower mosaic virus (CaMV)35S promoter and polyubiquitin (Ljubq1) promoter from Lotus japonicus using β-glucuronidase (gus) reporter gene in transgenic plants of L. japonicus. The promoter analysis demonstrated that the Ljubq1 promoter possessed higher activity than the CaMV35S promoter in leaves, stems, roots, nodules, and pollen. Finally, we created GATEWAY conversion technology-compatible binary vectors for over-expression and RNA interference under the Ljubq1 promoter. These materials could provide alternative choice for studies in L. japonicus.
ISSN:	0894-0282 1943-7706
DOI:	10.1094/mpmi-21-4-0375