Evaluation of the Estimation Capability of Response Surface Methodology and Artificial Neural Network for the Optimization of Bacteriocin-Like Inhibitory Substances Production by Lactococcus lactis Gh1

Bacteriocin-like inhibitory substances (BLIS) produced by Gh1 had shown antimicrobial activity against ATCC 15313. Brain Heart Infusion (BHI) broth is used for the cultivation and enumeration of lactic acid bacteria, but there is a need to improve the current medium composition for enhancement of BL...

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Veröffentlicht in:Microorganisms (Basel) 2021-03, Vol.9 (3), p.579
Hauptverfasser: Jawan, Roslina, Abbasiliasi, Sahar, Tan, Joo Shun, Kapri, Mohd Rizal, Mustafa, Shuhaimi, Halim, Murni, Ariff, Arbakariya B
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Sprache:eng
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Zusammenfassung:Bacteriocin-like inhibitory substances (BLIS) produced by Gh1 had shown antimicrobial activity against ATCC 15313. Brain Heart Infusion (BHI) broth is used for the cultivation and enumeration of lactic acid bacteria, but there is a need to improve the current medium composition for enhancement of BLIS production, and one of the approaches is to model the optimization process and identify the most appropriate medium formulation. Response surface methodology (RSM) and artificial neural network (ANN) models were employed in this study. In medium optimization, ANN (R = 0.98) methodology provided better estimation point and data fitting as compared to RSM (R = 0.79). In ANN, the optimal medium consisted of 35.38 g/L soytone, 16 g/L fructose, 3.25 g/L sodium chloride (NaCl) and 5.40 g/L disodium phosphate (Na HPO ). BLIS production in optimal medium (717.13 ± 0.76 AU/mL) was about 1.40-fold higher than that obtained in nonoptimised (520.56 ± 3.37 AU/mL) medium. BLIS production was further improved by about 1.18 times higher in 2 L stirred tank bioreactor (787.40 ± 1.30 AU/mL) as compared to that obtained in 250 mL shake flask (665.28 ± 14.22 AU/mL) using the optimised medium.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms9030579