Genome-Wide Identification and Characterization of GASA Gene Family in Nicotiana tabacum

The gibberellic acid stimulated Arabidopsis ( ) gene family is critical for plant growth, development, and stress response. gene family has been studied in various plant species, however, the gene family in tobacco ( ) have not been characterized in detail. In this study, we identified 18 genes in the tobacco genome, which were distributed to 13 chromosomes. All the proteins contained a conserved GASA domain and highly specific 12-cysteine residues at the C-terminus. Phylogenetic analysis divided the genes into three well-conserved subfamilies. Synteny analysis suggested that tandem and segmental duplications played an important role in the expansion of the gene family. -elements analysis showed that genes might influence different phytohormone and stress responses. Tissue expression analysis revealed that genes displayed unique or distinct expression patterns in different tissues, suggesting their potential roles in plant growth and development. We also found that the expression of genes were mostly regulated by abscisic and gibberellic acid, signifying their roles in the two phytohormone signaling pathways. Overall, these findings improve our understanding of genes and provided useful information for further studies on their molecular functions.